Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

A luminescent cocaine detection platform using a split G-quadruplex-selective iridium(III) complex and a three-way DNA junction architecture

Abstract

In this study, a series of 10 in-house cyclometalated iridium(III) complexes bearing different auxiliary ligands were tested for their selectivity toward split G-quadruplex in order to construct a label-free switch-on cocaine detection platform employing a three-way junction architecture and a G-quadruplex motif as a signal output unit. Through two rounds of screening, we discovered that the iridium(III) complex 7 exhibited excellent selectivity toward the intermolecular G-quadruplex motif. A detection limit as low as 30 nM for cocaine can be achieved by this sensing approach with a linear relationship between luminescence intensity and cocaine concentration established from 30 to 300 nM. Furthermore, this sensing approach could detect cocaine in diluted oral fluid. We hope that our simple, signal-on, label-free oligonucleotide-based sensing method for cocaine using a three-way DNA junction architecture could act as a useful platform in bioanalytical research.

Publication Year

2015

Journal Title

ACS Applied Materials and Interfaces

Volume number

7

Issue number

34

Publisher

American Chemical Society

First Page (page number)

19060

Last Page (page number)

19067

Referreed

1

DOI

10.1021/acsami.5b05861

ISSN (print)

19448244

Link to Publisher’s Edition

http://dx.doi.org/10.1021/acsami.5b05861

Keywords

cocaine, G-quadruplex, iridium(III) complex, label-free, three-way junction

This document is currently not available here.

Share

COinS