Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

Abstract

In this study, we synthesized a series of 9 luminescent iridium(III) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(III) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

Publication Year

2015

Journal Title

Chemical Communications

Volume number

51

Issue number

89

Publisher

Royal Society of Chemistry

First Page (page number)

16033

Last Page (page number)

16036

Referreed

1

DOI

10.1039/C5CC06655G

ISSN (print)

13597345

Link to Publisher’s Edition

http://dx.doi.org/10.1039/C5CC06655G

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