Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Development of an aptamer-based sensing platform for metal ions, proteins, and small molecules through terminal deoxynucleotidyl transferase induced G-quadruplex formation

Abstract

We report a label-free, structure-independent luminescent-sensing platform for metal ions, proteins, and small molecules utilizing an Ir(III) complex, terminal deoxynucleotidyl transferase (TdT), and a structure-folding aptamer. A novel G-quadruplex-selective Ir(III) complex was identified to detect the nascent G-quadruplex motifs with an enhanced luminescence response. Unlike most label-free DNA-based assays reported in the literature, this sensing platform does not require a specific secondary structure of aptamer, thus greatly simplifying DNA design. The detection platform was demonstrated by the detection of K+ ions, thrombin, and cocaine as representative examples of metal ions, proteins, and small molecules.

Publication Year

2015

Journal Title

ACS Applied Materials and Interfaces

Volume number

7

Issue number

43

Publisher

American Chemical Society

First Page (page number)

24046

Last Page (page number)

24052

Referreed

1

DOI

10.1021/acsami.5b08314

ISSN (print)

19448244

Link to Publisher’s Edition

http://dx.doi.org/10.1021/acsami.5b08314

Keywords

G-quadruplex, iridium(III) complex, label-free, TdT; universal detection

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