Document Type

Journal Article

Department/Unit

Department of Chemistry

Abstract

A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the construction of a label-free G-quadruplex-based adenosine detection assay in aqueous solution. Two different detection strategies were investigated for adenosine detection, and the results showed that initial addition of adenosine to the adenosine aptamer gave superior results. The assay exhibited a linear response for adenosine in the concentration range of 5 to 120 μM (R2 = 0.992), and the limit of detection for adenosine was 5 μM. Moreover, this assay was highly selective for adenosine over other nucleosides, and exhibited potential use for biological sample analysis.

Publication Year

2016

Journal Title

Scientific Reports

Volume number

6

Publisher

Nature Publishing Group

First Page (page number)

19368

Referreed

1

DOI

10.1038/srep19368

ISSN (print)

20452322

Link to Publisher’s Edition

http://dx.doi.org/10.1038/srep19368

Additional Files

JA-5034-28954_publisher_suppl.pdf (1220 kB)

Included in

Chemistry Commons

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