Document Type

Journal Article

Department/Unit

Department of Chemistry

Abstract

In this study, the reported G-quadruplex groove binder benzo[d,e]isoquinoline was linked to a cyclometallated Ir(III) complex to generate a highly selective DNA probe 1 that retains the favorable photophysical properties of the parent complex. The linked complex 1 showed advantages of both parent complex 2 and groove binder 3. Similar to 3, the conjugated complex 1 exhibits a superior affinity and selectivity for G-quadruplex DNA over other conformations of DNA or proteins, with the fold enhancement ratio obviously improved compared with parent complex 2. The molecular modelling revealed a groove-binding mode between complex 1 and G-quadruplex. Meanwhile 1 also possesses the prominent advantages of transition metal complex probes such as a large Stokes shift and long lifetime phosphorescence, which could be recognized in strong fluorescence media through time-resolved emission spectroscopy (TRES). We then employed 1 to develop a detection assay for AGR2, a potential cancer biomarker, as a “proof-of-principle” demonstration of the application of a linked complex for DNA-based detection in diluted fetal bovine serum. We anticipate that this conjugation method may be further employed in the development of DNA probes and have applications in label-free DNA-based diagnostic platforms.

Publication Year

2016

Journal Title

Chemical Science

Volume number

7

Issue number

4

Publisher

Royal Society of Chemistry

First Page (page number)

2516

Last Page (page number)

2523

Referreed

1

DOI

10.1039/C6SC00001K

ISSN (print)

20416520

Link to Publisher’s Edition

http://dx.doi.org/10.1039/C6SC00001K

Additional Files

JA-5034-28961_publisher_suppl.pdf (1116 kB)

Included in

Chemistry Commons

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