Document Type

Journal Article

Department/Unit

Department of Chemistry

Abstract

A novel Ir(III) complex 1 displays high selectivity for the G-quadruplex, and was used to establish a label-free G-quadruplex-based detection platform for lysozyme in buffer. In this study, we employed a special feature of most G-quadruplex probes that they do not respond towards the TBA G-quadruplex. A duplex DNA with a TBA G-quadruplex tail was designed for the detection of lysozyme. The presence of lysozyme will induce duplex dissociation and release the hybridized c-kit87 G-quadruplex, which would be recognized by the Ir(III) complex to generate a strong luminescence response. Common duplex DNA designs lacking a TBA G-quadruplex tail typically contain long complementary DNA regions that may be too stable to be dissociated, thus decreasing sensitivity. We anticipate that the presence of a TBA tail in the DNA duplex may destabilize the duplex structure, allowing the aptamer to be more easily dissociated and bind to the lysozyme, thereby increasing the sensitivity of the lysozyme detection platform. To our knowledge, this is the first example of the use of the TBA G-quadruplex to improve sensitivity through fine-tuning duplex stability. We believe that this approach may be further employed in sensing platforms for other targets. This assay exhibited a linear response for lysozyme within the concentration range of 2–50 nM (R2 = 0.9904), and the limit of detection for lysozyme was 2 nM. Moreover, this platform exhibited a potential use for biological sample analysis.

Publication Year

2016

Journal Title

Journal of Materials Chemistry B

Volume number

4

Issue number

14

Publisher

Royal Society of Chemistry

First Page (page number)

2407

Last Page (page number)

2411

Referreed

1

DOI

10.1039/C6TB00426A

ISSN (print)

2050750X

Link to Publisher’s Edition

http://dx.doi.org/10.1039/C6TB00426A

Included in

Chemistry Commons

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