Title

Quality assessment of Chinese medicines based on saccharide analysis

Year of Award

2014

Degree Type

Thesis

Degree Name

Master of Philosophy (MPhil)

Department

School of Chinese Medicine.

Principal Supervisor

Han, Simon Q. B.

Keywords

Analysis, China, Medicinal plants, Saccharides

Language

English

Abstract

Saccharides are the main constituents of many Chinese medicinal materials, they include monosaccharides, oligosaccharides and polysaccharides. They have been proved to have many bioactivities such as immunomodulating effects, anti-cancer effects, anti-viral activities, and anti-fatigue effects. Thereby, it is necessary to establish comprehensive qualitative and quantitative analysis of saccharides. On the other hand, saccharides are the main constituent of water extract of many Chinese medicines, which contains a large amount. Up to now, only two botanical drugs, namely Fulyzaq and Veregen, have been approved by the FDA. Despite their complex chemical profiles, 85%-95% of their chemical components could be determined, enabling effective quality control. Therefore, a higher level of qualitative and quantitative analysis is expected for saccharide-rich Chinese medicines. However, currently available methods are weak in terms of accuracy and specificity. Our study aims to develop a comprehensive qualitative and quantitative analysis of saccharides in saccharide-rich Chinese medicines. In this study, a HPLC-NH2P-ELSD quantification method is developed to determine monosaccharides and oligosaccharides. A high performance gel permeation chromatography method was developed to analyze the apparent molecular weight distribution patterns and a 3-Methyl-1-phenyl-2-pyrazoline-5-one derivatization method was used for monosaccharide composition analysis of polysaccharides. The methods were well validated and were then successfully applied in quality assessment of one Chinese medicine material and three Chinese patent drugs from different manufacturers.

Comments

Thesis (M.Phil.)--Hong Kong Baptist University, 2014.; Principal supervisor: Dr. Han Simon Q. B.;Thesis submitted to the School of Chinese Medicine.; Includes bibliographical references (pages 75-82)

This document is currently not available here.


Share

COinS