Year of Award

2016

Degree Type

Thesis

Degree Name

Master of Philosophy (MPhil)

Department

Department of Physics.

Principal Supervisor

Tang, Lei Han

Keywords

Biophysics, Cell adhesion, Cell proliferation, Cytology.

Language

English

Abstract

Cyst enlargement is an important part of the initial stage of organ formation. in vitro experiments have shown that the speed of expansion of this nearly spherical object is affected by many factors. Moreover, in many cases, bursting of the cyst cell layer takes place from time to time, leading to nearly periodic deflation of the cyst sphere. Biophysical models have been proposed that take into account the build-up of osmotic pressure in the lumen and cell proliferation in the cyst layer. Here we integrate two previous models for Hydra cyst swelling and collapse cycle and MDCK cyst growth saturation respectively to describe the Caco-2 cyst swelling and rupture cycle in a series of experiments carried out in Prof. Jian-Dong Huang’s lab at HKU. Gene expression analysis is also carried out to identify pathways that are over-expressed and active during cyst enlargement.

Comments

Principal supervisor: Prof. Tang Lei Han.;Thesis submitted to the Department of Physics. ; Thesis (M.Phil.)--Hong Kong Baptist University, 2015. ;

Bibliography

Includes bibliographical references (pages 47-50)

Copyright

The author retains all rights to this work. The author has signed an agreement granting HKBU a non-exclusive license to archive and distribute their thesis.



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