http://dx.doi.org/10.1016/B978-0-12-397926-1.00010-X">
 

Document Type

Book Chapter

Department/Unit

Department of Biology

Title

Chapter ten – cytokines, polarity proteins, and endosomal protein trafficking and signaling—the sertoli cell blood–testis barrier system in vitro as a study model

Language

English

Abstract

Endosomal signaling is emerging as one of the most important cellular events that regulate signaling function in mammalian cells or an epithelium in response to changes in environment such as the presence of stimuli mediated by cytokines, toxicants, heat, ions during growth and development, and other cellular processes such as cytokinesis and spermatogenesis. Recent studies have shown that protein endocytosis - the initial step of endosomal signaling - involves the participation of polarity proteins, such as partitioning defective protein 6 (Par6), Cdc42 and 14-3-3 (also known as Par5), which in turn is regulated by cytokines (e.g., TGF-β2, TGF-β3) and testosterone at the Sertoli cell blood-testis barrier (BTB) in the mammalian testis. In this short method paper, we provide a detailed protocol of assessing protein endocytosis, the initial and also the most critical step of endosomal signaling at the Sertoli cell BTB. This biochemical endocytosis assay summarizes our experience for the last decade, which should likely be performed in conjunction with the dual-labeled immunofluorescence analysis to assess protein endocytosis. While we are using a Sertoli cell in vitro system that mimics the BTB in vivo, this approach should be applicable to virtually all mammalian cells. © 2014 Elsevier Inc.

Keywords

Blood-testis barrier, Endocytosis, Endosome, Protein endocytosis, Recycling, Sertoli cell, Spermatogenesis, Testis, Transcytosis

Publication Date

2014

Source Publication Title

Methods in enzymology

Editors

Conn, P. Michael

Volume

534

Start Page

181

End Page

194

Series Title

Endosome Signaling Part A

Publisher

n.a.

ISBN (print)

9780123979261

This document is currently not available here.

Share

COinS