Document Type

Journal Article

Department/Unit

Department of Biology

Title

Dexamethasone (DEX) induces Osmotic stress transcription factor 1 (Ostf1) through the Akt-GSK3β pathway in freshwater Japanese eel gill cell cultures

Language

English

Abstract

© 2013. Published by The Company of Biologists Ltd. Osmosensing and osmoregulatory processes undertaken in gills of euryhaline fish are coordinated by integrative actions of various signaling molecules/transcriptional factors. Considerable numbers of studies report the hyper- and hypoosmoregulatory functions of fish gills, by illustrating the process of gill cell remodeling and the modulation of the expression of ion channels/transporters. Comparatively mechanistic information relayed from signal integration to transcriptional regulation in mediating gill cell functions has not yet been elucidated. In this study we demonstrate the functional links from cortisol stimulation, to Akt activation, to the expression of the transcriptional factor, Ostf1. Using the synthetic glucocorticoid receptor agonist, dexamethasone (DEX), Ostf1 expression is found to be activated via glucocorticoid receptor (GR) and mediated by the Akt-GSK3β signaling pathway. Pharmacological experiments using kinase inhibitors reveal that the expression of Ostf1 is negatively regulated by Akt activation. The inhibition of PI3K or Akt activities, by the specific kinase inhibitors (wortmannin, LY294002 or SH6), stimulates Ostf1 expression, while a reduction of GSK3β activity by LiCl reduces Ostf1 expression. Collectively, our report for the first time indicates that DEX can induce Ostf1 via GR, with the involvement of the Akt-GSK3β signaling pathway in primary eel gill cell cultures. The data also suggest that Ostf1 may play different roles in gill cell survival during seawater acclimation.

Keywords

Akt signaling, Dexamethasone, Eel gill cell culture, GSK3β, Osmotic stress transcription factor 1

Publication Date

2013

Source Publication Title

Biology Open

Volume

2

Start Page

487

End Page

491

Publisher

Company of Biologists

DOI

10.1242/bio.20134135

Link to Publisher's Edition

http://dx.doi.org/10.1242/bio.20134135

ISSN (print)

20466390

ISSN (electronic)

20466390

This document is currently not available here.

Share

COinS