Document Type

Journal Article

Department/Unit

Department of Biology

Title

iTRAQ-based proteomic profiling of the barnacle balanus amphitrite in response to the antifouling compound meleagrin

Language

English

Abstract

Marine biofouling refers to the unwanted accumulation of fouling organisms, such as barnacles, on artificial surfaces, resulting in severe consequences for marine industries. Meleagrin is a potential nontoxic antifoulant that is isolated from the fungus Penicillium sp.; however, its mechanistic effect mode of action on larval settlement remains unknown. Here, we applied iTRAQ coupled with 2D LC-MS/MS proteomic analysis to investigate the effect of meleagrin on the proteomic expression profile of cyprid development and aging in the barnacle Balanus amphitrite. Fifty proteins were differentially expressed in response to treatment with meleagrin, among which 26 proteins were associated with cyprid development/aging and 24 were specifically associated with the meleagrin treatment. The 66 proteins that were associated with aging only remained unaltered during exposure to meleagrin. Using KEGG analysis, those proteins were assigned to several groups, including metabolic pathways, ECM-receptor interactions, and the regulation of the actin cytoskeleton. Among the 24 proteins that were not related to the development/aging process, expression of the cyprid major protein (CMP), a vitellogenin-like protein, increased after the meleagrin treatment, which suggested that meleagrin might affect the endocrine system and prevent the larval molting cycle. With the exception of the chitin binding protein that mediates the molting process and ATPase-mediated energy processes, the majority of proteins with significant effects in previous studies in response to cyprid treatment with butenolide and polyether B remained unchanged in the present study, suggesting that meleagrin may exhibit a different mechanism. © 2013 American Chemical Society.

Keywords

antifouling, barnacle, biofouling, iTRAQ, larval settlement, meleagrin, proteome

Publication Date

2013

Source Publication Title

Journal of Proteome Research

Volume

12

Issue

5

Start Page

2090

End Page

2100

Publisher

American Chemical Society

DOI

10.1021/pr301083e

Link to Publisher's Edition

http://dx.doi.org/10.1021/pr301083e

ISSN (print)

15353893

ISSN (electronic)

15353907

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