Document Type

Journal Article

Department/Unit

Department of Biology

Title

Osmotic stress transcription factor 1b (Ostf1b) promotes migration properties with the modulation of epithelial mesenchymal transition (EMT) phenotype in human embryonic kidney cell

Language

English

Abstract

Osmoregulation is an essential mechanism for euryhaline fish. Gill cells undergo rapid mechanism to maintain the cellular homeostasis during osmotic stress. Reports have suggested that gill cells may be able to migrate between primary filament and secondary lamella during seawater acclimination. However, the factor that can trigger such process is not well-known. Previously, we identified the osmotic stress transcription factor 1b (Ostf1b) in medaka and found that it is an early hypertonic responsive gene and can activate the c-Jun N-terminal kinase (JNK) pathway. In this report, we aim to know if Ostf1b plays the role in the migration. Ostf1b was ectopic expressed in the human embryonic kidney cell line (HEK293) to understand the Ostf1b function. Results clearly demonstrated that Ostf1b could constitutively activate the Rho kinase 1 (ROCK1) and myosin light chain 2 (MLC2) signalling pathway that promotes cell migration, epithelial mesenchymal transition (EMT) and cytoskeletal dynamics through stress fibre formation. The study supports the notion of cell migration and cytoskeleton rearrangement theories in osmoregulation. © 2013 Elsevier Ltd. All rights reserved.

Keywords

Actin fibre, Cell migration, Epithelial mesenchymal transition, Osmotic, Stress transcription factor 1b, Tight junction

Publication Date

2013

Source Publication Title

International Journal of Biochemistry and Cell Biology

Volume

45

Issue

8

Start Page

1921

End Page

1926

Publisher

Elsevier

DOI

10.1016/j.biocel.2013.05.023

ISSN (print)

13572725

ISSN (electronic)

18785875

This document is currently not available here.

Share

COinS