http://dx.doi.org/10.1021/jp908418v">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

NAC-capped quantum dot as nuclear staining agent for living cells via an In Vivo steering strategy

Language

English

Abstract

We describe a new nuclear staining strategy by in vivo anchoring Hoechst 33342, a commonly used nuclear staining organic dye, onto water-soluble N-acetylcysteine (NAC)-capped CdTe quantum dots (QDs). The strong H-bonding and electrostatic interaction between the organic dye and the QDs entice the positively charged nuclear staining agent in carrying effectively the bright QDs into the nucleus of living cells. Dynamic confocal imaging studies indicated that all visible light emitting NAC-capped QDs with emitting wavelength ranging from 530 to 602 nm can be successfully targeted to the nucleus of the living cells within 30 min. The minimum quantity of Hoechst 33342 as the anchoring agent required in carrying QDs to the nucleus is found to be 5 μg/mL. In essence, a simple, fast, and specific bioimaging of the nucleus in living cells by QDs is accomplished. © 2010 American Chemical Society.

Publication Date

2010

Source Publication Title

Journal of Physical Chemistry C

Volume

114

Issue

4

Start Page

6216

End Page

6221

Publisher

American Chemical Society

ISSN (print)

19327447

ISSN (electronic)

19327455

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