Document Type

Journal Article

Department/Unit

Department of Biology

Title

Role of MicroRNA-214 in ginsenoside-Rg1-induced angiogenesis

Language

English

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional gene modulators. Ginsenoside-Rg1, one of the active components of ginseng, has been confirmed as an angiogenesis inducer. Using miRNA microarray analysis, a total of 17 (including miR-214) and 5 miRNAs were found to be down- or up-regulated by Rg1 in human umbilical vein endothelial cells (HUVECs), respectively. Since miR-214 is closely related to endothelial nitric oxide synthase (eNOS) and hence angiogenesis, its expression was further validated by qRT-PCR. We also investigated the role of miR-214 on eNOS expression and in tubulogenesis and motility of HUVEC by transfection of specific miRNA inhibitor or precursor. Our results suggested that Rg1 can down-regulate miR-214 expression in HUVEC, leading to an increase in eNOS expression, and in vitro cell migration and tube formation which can possibly promote angiogenesis. These results signify a new understanding towards how a simple natural compound can affect physiological changes through modulation of miRNA expression. © 2009 Elsevier B.V. All rights reserved.

Keywords

Angiogenesis, eNOS, Ginsenoside-Rg1, HUVEC, MicroRNA, miR-214

Publication Date

2009

Source Publication Title

European Journal of Pharmaceutical Sciences

Volume

38

Issue

4

Start Page

370

End Page

377

Publisher

Elsevier

DOI

10.1016/j.ejps.2009.08.008

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.ejps.2009.08.008

ISSN (print)

09280987

ISSN (electronic)

18790720

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