Document Type

Journal Article

Department/Unit

Department of Biology

Title

Generation of reactive oxygen species and oxidative stress in Escherichia coli and Staphylococcus aureus by a novel semiconductor catalyst

Language

English

Abstract

The objective of this study was to investigate antimicrobial mechanisms of a new catalytic material (charge transfer auto oxidation-reduction type catalyst, CT catalyst) that may have great potential for application in water/wastewater treatment. Generation of reactive oxygen species (ROS) in bacteria-free solution, induction of ROS and oxidative damage in bacteria (including E. coli and S. aureus) were examined for the CT catalyst. The results showed that significantly higher (p < 0.05, via t-test) amount of hydroxyl radicals was generated by the CT catalyst compared with the control, particularly after 6 h of contact time that more than twice of the amount of the control was produced. The generation of ROS in the bacteria was greater under higher pH and temperature levels, which closely related with the oxidative damage in cells. The results indicated that CT catalyst induced oxidative damage in the bacteria might serve as an important mechanism interpreting the anti-microbial function of the CT catalyst. © 2010 Springer Science+Business Media B.V.

Keywords

Antibacterial activity, Charge transfer auto oxidation-reduction type catalyst, Hydroxyl radicals, Lipid peroxidation, Protein oxidation, Superoxide anions

Publication Date

2011

Source Publication Title

Journal of Nanoparticle Research

Volume

13

Issue

3

Start Page

1007

End Page

1017

Publisher

Springer Verlag

DOI

10.1007/s11051-010-0128-7

Link to Publisher's Edition

http://dx.doi.org/10.1007/s11051-010-0128-7

ISSN (print)

13880764

ISSN (electronic)

1572896X

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