Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

Language

English

Abstract

We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(iii) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated. © 2013 The Royal Society of Chemistry.

Publication Date

2013

Source Publication Title

Chemical Communications

Volume

49

Issue

50

Start Page

5630

End Page

5632

Publisher

Royal Society of Chemistry

DOI

10.1039/c3cc41129j

Link to Publisher's Edition

http://dx.doi.org/10.1039/c3cc41129j

ISSN (print)

13597345

ISSN (electronic)

1364548X

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