Department of Chemistry
Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe
We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(iii) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated. © 2013 The Royal Society of Chemistry.
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
Leung, Ka-Ho, Hong-Zhang He, Victor Pui-Yan Ma, Hai-Jing Zhong, Daniel Shiu-Hin Chan, Jun Zhou, Jean-Louis Mergny, Chung-Hang Leung, and Dik-Lung Ma. "Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe." Chemical Communications 49.50 (2013): 5630-5632.