Document Type

Journal Article

Department/Unit

Department of Biology

Title

Polybrominated diphenyl ethers do not affect metamorphosis but alter the proteome of the invasive slipper limpet Crepidula onyx

Language

English

Abstract

Man-made polybrominated diphenyl ethers (PBDEs) used as flame retardants in various consumer products may be harmful to marine organisms. Larvae of some marine invertebrates, especially invasive species, can develop resistance to PBDEs through altered protein expression patterns or proteome plasticity. This is the first report of a proteomics approach to study BDE-47 induced molecular changes in the invasive limpet Crepidula onyx. Larvae of C. onyx were cultured for 5days (hatching to metamorphosis) in the presence of BDE-47 (1μgL-1). Using a 2-DE proteomics approach with triple quadrupole and high-resolution TOF-MS, we showed that BDE-47 altered the proteome structure but not the growth or metamorphosis of C. onyx larvae. We found eight significant differentially expressed proteins in response to BDE-47, deemed the protein expression signature, consisting of cytoskeletal, stress tolerance, metabolism and energy production related proteins. Our data suggest C. onyx larvae have adequate proteome plasticity to tolerate BDE-47 toxicity. © 2013 Elsevier Ltd.

Keywords

Crepidula onyx, Environmental proteomics, Larval metamorphosis, Polybrominated diphenyl ethers (PBDEs), Protein expression signatures

Publication Date

2013

Source Publication Title

Marine Pollution Bulletin

Volume

73

Issue

1

Start Page

273

End Page

281

Publisher

Elsevier

DOI

10.1016/j.marpolbul.2013.05.009

ISSN (print)

0025326X

ISSN (electronic)

18793363

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