Document Type

Journal Article

Department/Unit

Department of Biology

Title

PTHGRN: Unraveling post-translational hierarchical gene regulatory networks using PPI, ChIP-seq and gene expression data

Language

English

Abstract

Interactions among transcriptional factors (TFs), cofactors and other proteins or enzymes can affect transcriptional regulatory capabilities of eukaryotic organisms. Post-translational modifications (PTMs) cooperate with TFs and epigenetic alterations to constitute a hierarchical complexity in transcriptional gene regulation. While clearly implicated in biological processes, our understanding of these complex regulatory mechanisms is still limited and incomplete. Various online software have been proposed for uncovering transcriptional and epigenetic regulatory networks, however, there is a lack of effective web-based software capable of constructing underlying interactive organizations between post-translational and transcriptional regulatory components. Here, we present an open web server, post-translational hierarchical gene regulatory network (PTHGRN) to unravel relationships among PTMs, TFs, epigenetic modifications and gene expression. PTHGRN utilizes a graphical Gaussian model with partial least squares regression-based methodology, and is able to integrate protein-protein interactions, ChIP-seq and gene expression data and to capture essential regulation features behind high-throughput data. The server provides an integrative platform for users to analyze ready-to-use public high-throughput Omics resources or upload their own data for systems biology study. Users can choose various parameters in the method, build network topologies of interests and dissect their associations with biological functions. Application of the software to stem cell and breast cancer demonstrates that it is an effective tool for understanding regulatory mechanisms in biological complex systems. PTHGRN web server is publically available at web site http://www.byanbioinfo.org/pthgrn. © 2014 The Author(s).

Publication Date

2014

Source Publication Title

Nucleic Acids Research

Volume

42

Issue

W1

Start Page

W130

End Page

W136

Publisher

Oxford University Press

DOI

10.1093/nar/gku471

Link to Publisher's Edition

http://dx.doi.org/10.1093/nar/gku471

ISSN (print)

03051048

ISSN (electronic)

13624962

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