Document Type

Journal Article

Department/Unit

Department of Biology

Language

English

Abstract

Cell fate specification is typically initiated by a master regulator, which is relayed by tissue-specific regulatory proteins (usually transcription factors) for further enforcement of cell identities, but how the factors are coordinated among each other to "finish up" the specification remains poorly understood. Caenorhabditis elegans epidermis specification is initiated by a master regulator, ELT-1, that activates its targets, NHR-25 and ELT-3, two epidermis-specific transcription factors that are important for development but not for initial specification of epidermis, thus providing a unique paradigm for illustrating how the tissue-specific regulatory proteins work together to enforce cell fate specification. Here we addressed the question through contrasting genome-wide in vivo binding targets between NHR-25 and ELT-3. We demonstrate that the two factors bind discrete but conserved DNA motifs, most of which remain in proximity, suggesting formation of a complex between the two. In agreement with this, gene ontology analysis of putative target genes suggested differential regulation of metabolism but coordinated control of epidermal development between the two factors, which is supported by quantitative analysis of expression of their specific or common targets in the presence or absence of either protein. Functional validation of a subset of the target genes showed both activating and inhibitory roles of NHR-25 and ELT-3 in regulating their targets. We further demonstrated differential control of specification of AB and C lineage-derived epidermis. The results allow us to assemble a comprehensive gene network underlying C. elegans epidermis development that is likely to be widely used across species and provides insights into how tissue-specific transcription factors coordinate with one another to enforce cell fate specification initiated by its master regulator. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Published.

Keywords

C. elegans, Cell Differentiation, Chromatin Immunoprecipitation (ChIP), Epithelium, Gene Expression, Gene Regulation

Publication Date

11-2013

Source Publication Title

Journal of Biological Chemistry

Volume

288

Start Page

33411

End Page

33426

Publisher

American Society for Biochemistry and Molecular Biology

Peer Reviewed

1

Copyright

This research was originally published in Journal of Biological Chemistry. Shao, J., He, K., Wang, H., Ho, W., Ren, X., An, X., . . . Zhao, Z. Collaborative regulation of development but independent control of metabolism by two epidermis-specific transcription factors in caenorhabditis elegans. Journal of Biological Chemistry, 2013; 288(46): 33411-33426.© the American Society for Biochemistry and Molecular Biology.

Funder

This work was supported by the Collaborative Research Fund of the Research Grants Council in Hong Kong and a faculty research grant from Hong Kong Baptist University.

DOI

10.1074/jbc.M113.487975

Link to Publisher's Edition

http://dx.doi.org/10.1074/jbc.M113.487975

ISSN (print)

00219258

ISSN (electronic)

1083351X

Additional Files

JA-5189-25500_suppl.zip (184 kB)

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