http://dx.doi.org/10.1016/j.ymeth.2013.08.002">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Monitoring of DNA–protein interaction with single gold nanoparticles by localized scattering plasmon resonance spectroscopy

Language

English

Abstract

We reported a sensitive detection system for measuring DNA-protein interaction at single plasmonic metal nanoparticles level by Localized Scattering Plasmon Resonance (LSPR) spectroscopy. As a proof of concept, DNA molecules were conjugated to gold nanoparticles (AuNPs) through gold-thiol chemistry and the resulted complex was served as single-particle probes of human topoisomerase I (TOPO). By recording the changes in Rayleigh light scattering signal of the individual nanoparticles upon protein binding, DNA-protein interaction was monitored and measured. The λmax shifts in LSPR spectrum of individual AuNP was found to be highly correlated with the amount of TOPO that bound onto. This technique provides a sensitive and high-throughput platform to screen and monitor accurately the specific biomolecular interactions. It is capable of revealing information such as particle-particle variations that might be buried in conventional bulk measurement. © 2013 Elsevier Inc.

Keywords

LSPR, Protein-DNA interactions, Single nanoparticles

Publication Date

2013

Source Publication Title

Methods

Volume

64

Issue

3

Start Page

331

End Page

337

Publisher

Elsevier

ISSN (print)

10462023

ISSN (electronic)

10959130

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