Department of Chemistry
Monitoring of DNA–protein interaction with single gold nanoparticles by localized scattering plasmon resonance spectroscopy
We reported a sensitive detection system for measuring DNA-protein interaction at single plasmonic metal nanoparticles level by Localized Scattering Plasmon Resonance (LSPR) spectroscopy. As a proof of concept, DNA molecules were conjugated to gold nanoparticles (AuNPs) through gold-thiol chemistry and the resulted complex was served as single-particle probes of human topoisomerase I (TOPO). By recording the changes in Rayleigh light scattering signal of the individual nanoparticles upon protein binding, DNA-protein interaction was monitored and measured. The λmax shifts in LSPR spectrum of individual AuNP was found to be highly correlated with the amount of TOPO that bound onto. This technique provides a sensitive and high-throughput platform to screen and monitor accurately the specific biomolecular interactions. It is capable of revealing information such as particle-particle variations that might be buried in conventional bulk measurement. © 2013 Elsevier Inc.
LSPR, Protein-DNA interactions, Single nanoparticles
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Link to Publisher's Edition
Lo, Kin-Man, Chung-Yin Lai, Ho-Man Chan, Dik-Lung Ma, and Hung-Wing Li. "Monitoring of DNA–protein interaction with single gold nanoparticles by localized scattering plasmon resonance spectroscopy." Methods 64.3 (2013): 331-337.