Department of Chemistry
A label-free luminescent switch-on assay for atp using a g-quadruplex-selective iridium(III) complex
We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM. © 2013 Leung et al.
Source Publication Title
Public Library of Science
Link to Publisher's Edition
Leung, Ka-Ho, Lihua Lu, Modi Wang, Tsun-Yin Mak, Daniel Shiu-Hin Chan, Fung-Kit Tang, Chung-Hang Leung, Hiu-Yee Kwan, Zhiling Yu, and Dik-Lung Ma. "A label-free luminescent switch-on assay for atp using a g-quadruplex-selective iridium(III) complex." PLoS ONE 8.10 (2013).