Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

A label-free luminescent switch-on assay for atp using a g-quadruplex-selective iridium(III) complex

Language

English

Abstract

We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM. © 2013 Leung et al.

Publication Date

2013

Source Publication Title

PLoS ONE

Volume

8

Issue

10

Publisher

Public Library of Science

DOI

10.1371/journal.pone.0077021

ISSN (print)

19326203

ISSN (electronic)

19326203

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