Department of Chemistry
A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion
A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a luminescent probe in a label-free G-quadruplex-based detection assay for Ca2+ ions in aqueous solution. In this assay, a guanine-rich oligonucleotide (G4, 5'-G4T4G4-3') initially exists in an antiparallel G-quadruplex conformation, resulting in a low luminescence signal. Upon incubation with Ca2+ ions, the antiparallel G-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ ions with a limit of detection in the nanomolar range, and was selective for Ca2+ over other metal ions. © 2013 Elsevier Inc.
Ca2+ ions, G-quadruplex, Iridium, Luminescence, Metal complex
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Link to Publisher's Edition
He, Hong-Zhang, Modi Wang, Daniel Shiu-Hin Chan, Chung-Hang Leung, Xuexia Lin, Jin-Ming Lin, and Dik-Lung Ma. "A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion." Methods 64.3 (2013): 212-217.