http://dx.doi.org/10.1021/am404314p">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Label-free luminescent switch-on detection of endonuclease iv activity using a G-quadruplex-selective iridium(III) complex

Language

English

Abstract

We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy) 2(bcp)]+ (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes. © 2013 American Chemical Society.

Keywords

AP endonuclease, G-quadruplex, iridium(III) complex, label-free, luminescent

Publication Date

2013

Source Publication Title

ACS Applied Materials and Interfaces

Volume

5

Issue

23

Start Page

12249

End Page

12253

Publisher

American Chemical Society

ISSN (print)

19448244

ISSN (electronic)

19448252

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