Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

Language

English

Abstract

A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity. © the Partner Organisations 2014.

Publication Date

2014

Source Publication Title

Chemical Communications

Volume

50

Issue

40

Start Page

5313

End Page

5315

Publisher

Royal Society of Chemistry

DOI

10.1039/C3CC47444E

Link to Publisher's Edition

http://dx.doi.org/10.1039/C3CC47444E

ISSN (print)

13597345

ISSN (electronic)

1364548X

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