Department of Chemistry
Luminescent detection of human serum albumin in aqueous solution using a cyclometallated iridium(III) complex
A novel cyclometalated iridium(III) complex has been developed as a luminescent probe for human serum albumin (HSA) detection in aqueous solution. In this system, the initial luminescence of the iridium(III) complex in the absence of human serum albumin is weak. However, the addition of HSA could greatly enhance the luminescence intensity of iridium(III) complex. The assay could detect down to 0.05 μM of HSA, and also exhibited good selectivity for HSA over bovine serum albumin and other biomolecules such as ATP, β-amyloid, peroxidase, thrombin, lysozyme, trypsin, hemoglobin, and the various nucleic acids. Furthermore, the application of the assay for the detection of HSA in diluted urine samples and for monitoring the conformational changes of HSA induced by exogenous protein unfolding agents was also demonstrated. © 2014 Elsevier B.V.
Human serum albumin, Iridium(III) complex, Luminescence, Probe
Source Publication Title
Sensors and Actuators B: Chemical
Link to Publisher's Edition
Lu, Lihua, Hong-Zhang He, Hai-Jing Zhong, Li-Juan Liu, Daniel Shiu-Hin Chan, Chung-Hang Leung, and Dik-Lung Ma. "Luminescent detection of human serum albumin in aqueous solution using a cyclometallated iridium(III) complex." Sensors and Actuators B: Chemical 201 (2014): 177-184.