http://dx.doi.org/10.1016/j.ibiod.2013.11.005">
 

Document Type

Journal Article

Authors

Haijun Liu, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Jun Yao, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Zhimin Yuan, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Yifan Shang, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Huilun Chen, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Fei Wang, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Kanaji Masakorala, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Chan Yu, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Minmin Cai, School of Civil and Environmental Engineering, National International Cooperation Base on Environment and Energy, University of Science and Technology Beijing
Ruth E. Blake, Department of Geology and Geophysics, Yale University
Martin M.F. Choi, Department of Chemistry, Hong Kong Baptist UniversityFollow

Department/Unit

Department of Chemistry

Title

Isolation and characterization of crude-oil-degrading bacteria from oil-water mixture in Dagang oilfield, China

Language

English

Abstract

Isolating novel crude-oil-degrading bacteria from oil-water mixture of oil production well and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude oil exploitation. According to the molecular screening with degenerate primers of alkane hydroxylase gene (alk B), a strain Acinetobacter sp. LS-1 with alk B gene was isolated. This strain exhibited a 99.9% similarity with genus Acinetobacter. This alk B gene which is one of the key enzymes of metabolic process was identified. This gene sequence showed 98% similarity of its nucleotide and related amino acids to the genus Marinobacter but exhibited below 70% similarity to the genus Acinetobacter. This phylogenetic analysis indicated that alk B may have been transferred horizontally between bacteria. The isolated strain could utilize crude oil as the sole carbon, achieving a high degradation (70.3%) in 7 days. Microcalorimetric analysis of the metabolic processes for hexadecane degradation also demonstrated the ability of this strain to utilize hydrocarbons. Thus, this strain enables to degrade hydrocarbons as the sole carbon source from the gene level, combined with material and energy metabolism. These findings will benefit this strain in the remediation of oil-polluted environments and oil exploitation. © 2013 Elsevier Ltd.

Keywords

Acinetobacter, Alkane hydroxylase gene, Crude oil, Degradation, Thermal effect

Publication Date

2014

Source Publication Title

International Biodeterioration and Biodegradation

Volume

87

Start Page

52

End Page

59

Publisher

Elsevier

ISSN (print)

09648305

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