http://dx.doi.org/10.1039/c4ob00382a">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

A fluorescent probe for intracellular cysteine overcoming the interference by glutathione

Language

English

Abstract

Cysteine (Cys) plays important roles in many physiological processes of eukaryotic cells and its detection in cells is of fundamental significance. However, glutathione (GSH), homocysteine, N-acetyl-l-cysteine and other thiols greatly hamper the detection of Cys. In particular, GSH strongly interferes with the detection of cellular Cys (30-200 μM) due to its high intracellular concentration (1-10 mM). In this work, an off-on fluorescent probe (HOTA) for the detection of Cys is presented. This probe possesses both excellent sensitivity and satisfactory selectivity for cellular Cys detection: with the addition of 200 μM Cys, the fluorescence intensity of the probe (10 μM) enhanced 117-fold and the detection limit was calculated to be 13.47 μM, which is lower than the cellular Cys concentration; the probe also selectively detected 30-200 μM cysteine over 1-10 mM glutathione. Consequently, cell imaging experiments were performed with probe HOTA. Furthermore, the results of the thiol-blocking and GSH synthesis inhibiting experiments confirmed that the intracellular emission mainly originates from the interaction between Cys and HOTA. This journal is © the Partner Organisations 2014.

Publication Date

2014

Source Publication Title

Organic and Biomolecular Chemistry

Volume

12

Issue

32

Start Page

6128

End Page

6133

Publisher

Royal Society of Chemistry

ISSN (print)

14770520

ISSN (electronic)

14770539

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