Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Proteome profiling of spinal cord and dorsal root ganglia in rats with trinitrobenzene sulfonic acid-induced colitis

Language

English

Abstract

AIM: To investigate proteomic changes in spinal cord and dorsal root ganglia (DRG) of rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis. METHODS: The colonic myeloperoxidase (MPO) activity and tumor necrosis factor-α (TNF-α) level were determined. A two-dimensional electrophoresis (2-DE)- based proteomic technique was used to profile the global protein expression changes in the DRG and spinal cord of the rats with acute colitis induced by intracolonic injection of TNBS. RESULTS: TNBS group showed significantly elevated colonic MPO activity and increased TNF-α level. The proteins derived from lumbosacral enlargement of the spinal cord and DRG were resolved by 2-DE; and 26 and 19 proteins that displayed significantly different expression levels in the DRG and spinal cord were identified respectively. Altered proteins were found to be involved in a number of biological functions, such as inflammation/immunity, cell signaling, redox regulation, sulfate transport and cellular metabolism. The overexpression of the protein similar to potassium channel tetramerisation domain containing protein 12 (Kctd 12) and low expression of proteasome subunit α type-1 (psma) were validated by Western blotting analysis. CONCLUSION: TNBS-induced colitis has a profound impact on protein profiling in the nervous system. This result helps understand the neurological pathogenesis of inflammatory bowel disease. © 2012 Baishideng. All rights reserved.

Keywords

Dorsal root ganglia, Inflammatory bowel disease, Spinal cord, Trinitrobenzene sulfonic acid, Two-dimensional electrophoresis-based proteomic technique

Publication Date

2012

Source Publication Title

World Journal of Gastroenterology

Volume

18

Issue

23

Start Page

2914

End Page

2928

Publisher

Baishideng Publishing Group Co. Limited

DOI

10.3748/wjg.v18.i23.2914

Link to Publisher's Edition

http://dx.doi.org/10.3748/wjg.v18.i23.2914

ISSN (print)

10079327

ISSN (electronic)

22192840

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