Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

An interesting two-phase solvent system and its use in preparative isolation of aconitines from aconite roots by counter-current chromatography

Language

English

Abstract

Two-phase solvent system plays crucial role in successful separation of organic compounds using counter-current chromatography (CCC). An interesting two-phase solvent system, composed of chloroform/ethyl acetate/methanol/water, is reported here, in which both phases contain sufficient organic solvents to balance their dissolving capacities. Adjusting the solvent system to get satisfactory partition coefficients (K values) for target compounds becomes relatively simple. This solvent system succeeded in sample preparation of aconitine (8.07 mg, 93.69%), hypaconitine (7.74 mg, 93.17%), mesaconitine (1.95 mg, 94.52%) from raw aconite roots (102.24 mg, crude extract), benzoylmesaconine (34.79 mg, 98.67%) from processed aconite roots (400.01 mg, crude extract), and yunaconitine (253.59 mg, 98.65%) from a crude extract of Aconitum forrestii (326.69 mg, crude extract). © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

Aconitine, Counter-current chromatography, Two-phase solvent system

Publication Date

2013

Source Publication Title

Journal of Separation Science

Volume

36

Issue

7

Start Page

1304

End Page

1310

Publisher

Wiley-VCH Verlag

DOI

10.1002/jssc.201201079

Link to Publisher's Edition

http://dx.doi.org/10.1002/jssc.201201079

ISSN (print)

16159306

ISSN (electronic)

16159314

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