Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

Background

The root of Fordia cauliflora Hemsl (FC) has long been used in southern China for the treatment of rheumatism, bruises, dementia in children, and valetudinarianism. However, sometimes it is mixed with other parts. And it has always been confused with the root of Millettia pulchra (Benth.) Kurz var. laxior(Dunn) Z. Wei (MP) by the local people. The chemical differences between the two ethnic drugs are not clear until now. The aim of this study is to develop a precise and accurate method to characterize and quantify multiple chemical components of FC, which is helpful for the quality evaluation and identification of FC.

Results

A method coupling ultra performance liquid chromatography (UPLC) with triple-quadrupole mass spectrometry (QqQ-MS) was first developed for simultaneous determination of five flavonoids in different parts of FC and the root of MP, based on a UPLC-diode array detection (DAD) fingerprint method. All calibration curves showed good linearity (R2>0.99) within test ranges. The overall LOD and LOQ were lower than 2.5 ng/mL and 5.0 ng/mL, respectively. The RSDs for intra- and inter-day of five analytes were less than 2.83% and 3.04%, respectively. Recovery studies for the quantified compounds were found to be within the range 93.6-99.8% with RSD less than 5.73%. The results suggest that the root, traditionally used medicinal part, yields the highest flavanoid content in FC. Pachycarin A, 3′,4′-dimethoxy(2′′,3′′:7,8) furanoflavone, karanjachromene and isoderricin A can be used to differentiate between FC and MP samples.

Conclusions

The present method is specific, precise and reliable, and is suitable for characterizing and quantifying multiple chemical components of FC.

Keywords

Determination, Ethnic drug, Fordia cauliflora, Fordiae Cauliforae Radix, Identification, Millettia pulchra var. laxior, Millettiae Pulchrae Radix, UPLC-DAD, UPLC-QqQ-MS

Publication Date

7-2013

Source Publication Title

Chemistry Central Journal

Volume

7

Start Page

126

Publisher

Chemistry Central

Peer Reviewed

1

Copyright

© Fan et al.; licensee Chemistry Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Funder

This research was financially supported by the Health Department of Guangxi province (Zhong2010111, Z2010175), and Guangxi Administration of Traditional Chinese Medicine (GZKZ1131).

DOI

10.1186/1752-153X-7-126

Link to Publisher's Edition

http://dx.doi.org/10.1186/1752-153X-7-126

ISSN (print)

1752153X

ISSN (electronic)

1752153X

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