Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Profiling of secondary metabolites in tissues from Rheum palmatum L. using laser microdissection and liquid chromatography mass spectrometry

Language

English

Abstract

Evaluating the quality of herbal medicines by morphological features is a convenient, quick, and practical method compared with other methods that mostly depend on modern instruments. Here, laser microdissection and ultraperformance liquid chromatography are combined with mass spectrometry to map the distribution of secondary metabolites in cells or tissues of a herb itself for correlating its bioactive components and morphological features. The root and rhizome of Rheum palmatum L. were taken as research target, which is the Chinese medicine, Radix et Rhizoma Rhei. According to fluorescent microscopic characteristics, 12 herbal cells or tissues of Radix et Rhizoma Rhei were separated by laser microdissection. Thirty-eight compounds were identified or tentatively characterized in the microdissected tissues. (+)-Catechin, 1-O-galloyl-2-Ocinnamoyl- β-D-glucose, and emodin were found to be the major components in most of the tissues. The brown ergastic substances found in rays of normal and anomalous vascular bundles as well as the parenchymatous cells of rhizome pith and the parenchymatous cells of root xylem contained higher than average amounts of these three components and more kinds of secondary metabolites. Overall, results suggest that Radix et Rhizoma Rhei of larger size and with conspicuous brocaded patterns and star spots are of higher quality as they tend to have greater contents of bioactive components. The study provides quantitative and specific criteria by which the quality of Radix et Rhizoma Rhei can be judged. This research also established a new, reliable, and practical method for direct profiling and imaging of secondary metabolites in any herbal tissue. © Springer-Verlag Berlin Heidelberg 2012.

Keywords

Herbal tissues, Laser microdissection, Liquid chromatography-mass spectrometry, Rheum palmatum L., Secondary metabolites

Publication Date

2013

Source Publication Title

Analytical and Bioanalytical Chemistry

Volume

405

Issue

12

Start Page

4199

End Page

4212

Publisher

Springer Verlag

DOI

10.1007/s00216-013-6819-z

Link to Publisher's Edition

http://dx.doi.org/10.1007/s00216-013-6819-z

ISSN (print)

16182642

ISSN (electronic)

16182650

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