Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Molecular analysis of stemona plants in China based on sequences of four chloroplast DNA regions

Language

English

Abstract

Stemona sessilifolia, S. japonica and S. tuberosa are the three original sources of Stemonae Radix specified in the Chinese Pharmacopoeia (CP), and have been traditionally used for antitussive and insecticidal remedy. Significant variations in alkaloids composition and content, as well as different degrees of antitussive activities were found among them. In order to identify the genuine sources of Stemonae Radix accurately in genetic level, we determined the nucleotide sequences of chloroplast DNA trnL-trnF, trnH-psbA, petB-petD and trnK-rps16 regions of the species recorded in CP and S. parviflora, as well as the common counterfeits of Stemonae Radix, Asparagus species. The results revealed that the sequences of petB-petD and trnK-rps16 regions, showing relatively high substitution rate, were more informative than those of trnL-trnF and trnH-psbA regions. The sequences from all the four regions provided useful information to discriminate the three CP species from each other and from S. parviflora and the counterfeits. A phylogenetic tree reconstructed by the trnH-psbA sequences for 9 Stemona species distributed in China and Thailand showed that the three CP species belonged to the same clade, among which S. japonica and S. sessillifolia formed a sister group, showing closer relations to each other than to S. tuberosa. By contrast, S. parviflora was genetically far from the three CP species. Intra-species variations were observed in the three CP species. Especially, in S. tuberosa two types of petB-petD sequence and four types each of trnL-trnF, trnK-rps16 and trnH-psbA sequences resulted in 6 haplotypes; whereas, these differences had no relation with the different chemical types, but seemed to be consistent with geographical distribution. © 2009 Pharmaceutical Society of Japan.

Keywords

Molecular identification, petB-petD, Stemona, trnH-psbA, trnK-rps16, trnL-trnF

Publication Date

2009

Source Publication Title

Biological and Pharmaceutical Bulletin

Volume

32

Issue

8

Start Page

1439

End Page

1446

Publisher

Pharmaceutical Society of Japan

DOI

10.1248/bpb.32.1439

Link to Publisher's Edition

http://dx.doi.org/10.1248/bpb.32.1439

ISSN (print)

09186158

ISSN (electronic)

13475215

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