http://dx.doi.org/10.1002/ptr.2940">
 

Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Neuroprotective effect of luteolin on amyloid β protein (25–35)-induced toxicity in cultured rat cortical neurons

Language

English

Abstract

The present study was carried out to investigate the neuroprotective effect of luteolin on amyloid β (Aβ) (25-35)-induced neurotoxicity using cultured rat cortical neurons. After exposure of primary cultures of rat cortical cells to 10 μM Aβ (25-35) for 48 h, cortical cell cultures exhibited marked apoptotic death. Pretreatment with luteolin (1, 10 μM) significantly protected cortical cell cultures against Aβ (25-35)-induced toxicity. Luteolin (1, 10 μM) showed a concentration-dependent inhibition on 10 μM Aβ (25-35)-induced apoptotic neuronal death, as assessed by MTT assay. Furthermore, luteolin reduced apoptotic characteristics by DAPI staining. For Western blot analysis, the results showed that the protective effect of luteolin on Aβ (25-35)-induced neurotoxicity was mediated by preventing of ERK-p, JNK, JNK-p, P38-p and caspase 3 activations in rat primary cortical cultures. Taken together, the results suggest that luteolin prevents Aβ (25-35)-induced apoptotic neuronal death through inhibiting the protein level of JNK, ERK and p38 MAP kinases and caspase 3 activations. Copyright © 2009 John Wiley & Sons, Ltd.

Keywords

Aβ, Caspase 3, Cortical neurons, Luteolin, MAP kinases

Publication Date

2010

Source Publication Title

Phytotherapy Research

Volume

24

Issue

S1

Start Page

S102

End Page

S108

Publisher

Wiley

ISSN (print)

0951418X

ISSN (electronic)

10991573

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