Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Chemical and DNA authentication of taste variants of Gynostemma pentaphyllum herbal tea

Language

English

Abstract

Gynostemma pentaphyllum Makino (Gp) was once used as a sweetener in Japan and is now widely consumed as an herbal tea worldwide for lowering cholesterol levels. Two taste variants, bitter and sweet, of Gp exist in the commercial market, but they cannot be differentiated morphologically nor by existing chemical analytical methods. This has been creating a problem in quality control of Gp products. In the present study, using HPLC-DAD and HPLC-ESI-MS analysis, we found that the Gp saponins, not flavonoids, from the sweet and bitter variants have distinctly different profiles. In addition, the two variants share only 69.01% homology in the ribosomal ITS-1 region, suggesting a phylogenic gap between these two variants. The combinations of chemical profiling and phylogenic analysis clearly confirm, for the first time, the distinction between these two taste variants. This information has direct application in the authentication and quality assessment of the various Gynostemma tea products. © 2011 Elsevier Ltd. All rights reserved.

Keywords

Gynostemma pentaphyllum, Gypenoside, ITS sequence, Phylogeny, Taste variants

Publication Date

2011

Source Publication Title

Food Chemistry

Volume

128

Issue

1

Start Page

70

End Page

80

Publisher

Elsevier

DOI

10.1016/j.foodchem.2011.02.078

ISSN (print)

03088146

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