School of Chinese Medicine
Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography
In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine: water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another. © 2011 The Japanese Society of Pharmacognosy and Springer.
Ephedra alkaloids, Ephedra Herb, Mahuang, Simultaneous determination, Species identification
Source Publication Title
Journal of Natural Medicines
Hong, Hao, Hu-Biao Chen, Dong-Hui Yang, Ming-Ying Shang, Xuan Wang, Shao-Qing Cai, and Masayuki Mikage. "Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography." Journal of Natural Medicines 65.4-3 (2011): 623-628.