School of Chinese Medicine
Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion
Euphorbia factor L1 (EFL1) belongs to diterpenoids of genus Euphorbia. In this article, its reversal activity against ABCB1-mediated MDR in KBv200 and MCF-7/adr cells was reported. However, EFL1 did not alter the sensitivity of KB and MCF-7 cells to chemotherapeutic agents. Meanwhile, EFL1 significantly increased accumulation of doxorubicin and rhodamine 123 in KBv200 and MCF-7/adr cells, showing no siginficant influence on that of KB and MCF-7 cells. Furthermore, EFL1 could enhance the ATP hydrolysis activity of ABCB1 stimulated by verapamil. At the same time, EFL1 inhibited the efflux of ABCB1 in KBv200 and MCF-7/adr cells. In addition, EFL1 did not downregulate expression of ABCB1 in KBv200 and MCF-7/adr cells either in mRNA or protein level. © 2011 Wiley-Liss, Inc.
ABCB1, Euphorbia factor L1, Multidrug resistance
Source Publication Title
Journal of Cellular Biochemistry
Zhang, Jian-Ye, Yan-Jun Mi, Shu-Peng Chen, Fang Wang, Yong-Ju Liang, Li-Sheng Zheng, Cheng-Jun Shi, Li-Yang Tao, Li-Ming Chen, Hu-Biao Chen, and Li-Wu Fu. "Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion." Journal of Cellular Biochemistry 112.4 (2011): 1076-1083.