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Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Histochemical analysis of the root tuber of Polygonum multiflorum Thunb. (Fam. Polygonaceae)

Language

English

Abstract

Authentication is the first priority in quality evaluation of Chinese herbal medicines (CHMs). The most commonly used authentication methods are morphological identification and microscopic identification. Unfortunately, these two methods cannot provide the chemical information needed to assess the quality of CHMs. In this study, a combination of fluorescence microscopy and high performance liquid chromatography-mass spectrometry (HPLC-MS) was used to analyze the chemical profiles of the tissues of the raw root tubers of Polygonum multiflorum Thunb. The results showed that the cork cells, cortex, and vessels in transverse sections of the raw root tuber of P. multiflorum fluoresced differently. Further analysis by HPLC-MS revealed that anthraquinones are mainly distributed in the cortex, and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside could be found in all tissues of the raw root tubers of P. multiflorum with its relative content as cork > cortex > xylem of allotype vascular bundles > xylem of central vascular bundles. Moreover, the fluorescence characteristics of the tissues from the steamed root tuber of P. multiflorum were compared and showed different fluorescence from those of raw material. From these results, it can be deduced that the root tuber of P. multiflorum with a broader cortex and fewer vascular bundles visible in a transverse section should be of better quality. The different fluorescence characteristics can be used to differentiate raw root tubers of P. multiflorum from those that have been steamed. © 2010 Wiley-Liss, Inc.

Keywords

Anthraquinone, Fluorescence microscopy, Histochemical analysis, Polygonum multiflorum Thunb

Publication Date

2011

Source Publication Title

Microscopy Research and Technique

Volume

74

Issue

6

Start Page

488

End Page

495

Publisher

Wiley

ISSN (print)

1059910X

ISSN (electronic)

10970029

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