http://dx.doi.org/10.1016/j.taap.2012.04.021">
 

Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Eriocalyxin B induces apoptosis and cell cycle arrest in pancreatic adenocarcinoma cells through caspase- and p53-dependent pathways

Language

English

Abstract

Pancreatic cancer is difficult to detect early and responds poorly to chemotherapy. A breakthrough in the development of new therapeutic agents is urgently needed. Eriocalyxin B (EriB), isolated from the Isodon eriocalyx plant, is an ent-kaurane diterpenoid with promise as a broad-spectrum anti-cancer agent. The anti-leukemic activity of EriB, including the underlying mechanisms involved, has been particularly well documented. In this study, we demonstrated for the first time EriB's potent cytotoxicity against four pancreatic adenocarcinoma cell lines, namely PANC-1, SW1990, CAPAN-1, and CAPAN-2. The effects were comparable to that of the chemotherapeutic camptothecin (CAM), but with much lower toxicity against normal human liver WRL68 cells. EriB's cytoxicity against CAPAN-2 cells was found to involve caspase-dependent apoptosis and cell cycle arrest at the G2/M phase. Moreover, the p53 pathway was found to be activated by EriB in these cells. Furthermore, in vivo studies showed that EriB inhibited the growth of human pancreatic tumor xenografts in BALB/c nude mice without significant secondary adverse effects. These results suggest that EriB should be considered a candidate for pancreatic cancer treatment. © 2012 Elsevier Inc.

Keywords

Apoptosis, Cell cycle arrest, Eriocalyxin B, P53, Pancreatic cancer

Publication Date

2012

Source Publication Title

Toxicology and Applied Pharmacology

Volume

262

Issue

1

Start Page

80

End Page

90

Publisher

Elsevier

ISSN (print)

0041008X

ISSN (electronic)

10960333

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