Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Studying effects of Magnolol on alpha-particle induced bystander effects using PADC-film based dishes

Language

English

Abstract

Radiation-induced bystander effect refers to the biological response found in cells (called bystander cells) which are not irradiated directly by ionizing radiation but are next to cells irradiated directly by ionizing radiation. In the present paper, the effects of Magnolol, an extract from the bark of Magnolia officinalis which is used as a traditional Chinese medicine, were studied on alpha-particle induced bystander effects. In our experiments, Chinese hamster ovary (CHO) cells were cultured in PADC-film based dishes and were irradiated with low fluences of alpha particles passing through the PADC films. The precise number of cells traversed or missed by alpha particles could be determined by studying the alpha-particle tracks developed on the PADC films upon subsequent chemical etching. TdT-mediated dUTP Nick-End Labeling (TUNEL) assay was employed to analyze the biological response of bystander cells in terms of DNA strand breaks. With the pretreatment of Magnolol, the DNA strand breaks in bystander cells were reduced, which showed that the alpha-particle induced bystander effects were suppressed with the presence of Magnolol. Since Magnolol is an antioxidant which can scavenge reactive oxygen species (ROS), our results give support to that ROS play a role in the bystander signal transmission in our experiments. © 2009 Elsevier Ltd. All rights reserved.

Keywords

Alpha-particle, Bystander effect, CHO cells, Magnolol, PADC

Publication Date

2009

Source Publication Title

Radiation Measurements

Volume

44

Issue

10-9

Start Page

1081

End Page

1084

Publisher

Elsevier

DOI

10.1016/j.radmeas.2009.10.029

ISSN (print)

13504487

This document is currently not available here.

Share

COinS