School of Chinese Medicine
A herbal formula consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages
Ethnopharmacological relevance A herbal formula (RL) consisting of Rosae Multiflorae Fructus (Yingshi) and Lonicerae Japonicae Flos (Jinyinhua) has been traditionally used to treat inflammatory disorders. This study aims to investigate the anti-inflammatory mode and mechanism of action of the ethanol extract of RL so as to provide a pharmacological basis for the use of RL in treating inflammatory diseases. Materials and method RL consisting of Yingshi and Jinyinhua (in 5:3 ratio) was extracted using absolute ethanol. We investigated its effects on nitric oxide (NO), interleukin-6 (IL-6), tumour necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinases (MAPKs) and nuclear factor κB (NFκB) in mouse RAW 264.7 macrophages activated with lipopolysaccharide (LPS). Results RL could decrease the secretion of NO, IL-6 and TNF-α into the culture medium and the cellular protein levels of iNOS and COX-2, which were associated with the reduction of the phosphorylation/ activation of JNK and p38, and the inhibition of the transcriptional activity of NF-κB. Conclusions The present study demonstrated an inhibitory effect of RL on the inflammatory mediators regulated by the NF-κB and MAPK signalling pathways in LPS-stimulated RAW 264.7 macrophages, providing a pharmacological basis for RL in the control of inflammatory disorders. © 2014 Elsevier Ireland Ltd.
Anti-inflammation, Lonicera japonica, MAPK, NF-κB, Rosa multiflora
Source Publication Title
Journal of Ethnopharmacology
Cheng, Brian Chi-Yan, Xiao-Qing Ma, Hiu-Yee Kwan, Kai-Wing Tse, Hui-Hui Cao, Tao Su, Xin Shu, Zheng-Zhi Wu, and Zhi-Ling Yu. "A herbal formula consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages." Journal of Ethnopharmacology 153.3 (2014): 922-927.