http://dx.doi.org/10.1016/j.jep.2014.02.029">
 

Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

A herbal formula consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages

Language

English

Abstract

Ethnopharmacological relevance A herbal formula (RL) consisting of Rosae Multiflorae Fructus (Yingshi) and Lonicerae Japonicae Flos (Jinyinhua) has been traditionally used to treat inflammatory disorders. This study aims to investigate the anti-inflammatory mode and mechanism of action of the ethanol extract of RL so as to provide a pharmacological basis for the use of RL in treating inflammatory diseases. Materials and method RL consisting of Yingshi and Jinyinhua (in 5:3 ratio) was extracted using absolute ethanol. We investigated its effects on nitric oxide (NO), interleukin-6 (IL-6), tumour necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinases (MAPKs) and nuclear factor κB (NFκB) in mouse RAW 264.7 macrophages activated with lipopolysaccharide (LPS). Results RL could decrease the secretion of NO, IL-6 and TNF-α into the culture medium and the cellular protein levels of iNOS and COX-2, which were associated with the reduction of the phosphorylation/ activation of JNK and p38, and the inhibition of the transcriptional activity of NF-κB. Conclusions The present study demonstrated an inhibitory effect of RL on the inflammatory mediators regulated by the NF-κB and MAPK signalling pathways in LPS-stimulated RAW 264.7 macrophages, providing a pharmacological basis for RL in the control of inflammatory disorders. © 2014 Elsevier Ireland Ltd.

Keywords

Anti-inflammation, Lonicera japonica, MAPK, NF-κB, Rosa multiflora

Publication Date

2014

Source Publication Title

Journal of Ethnopharmacology

Volume

153

Issue

3

Start Page

922

End Page

927

Publisher

Elsevier

ISSN (print)

03788741

ISSN (electronic)

18727573

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