http://dx.doi.org/10.1055/s-0033-1350708">
 

Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Triptolide downregulates treg cells and the level of IL-10, TGF-β, and VEGF in melanoma-bearing mice

Language

English

Abstract

Regulatory T cells play a key role in suppressing tumor immunity. Triptolide, a major active component isolated from the Chinese medicinal herb Tripterygium wilfordii, has been proven to possess multiple antitumor activities. Here, we investigated the effect of triptolide on regulatory T cells and on the level of IL-10, transforming growth factor-β, and vascular endothelial growth factor in tumor-bearing mice. Fifty male C57BL/6 mice were randomly grouped as follows: normal control group, model group with B16-F10 cells implanted, and three treatment groups with cyclophosphamide, triptolide-high dose, triptolide-low dose. The proportion of regulatory T cells in the spleen and axillary lymph nodes was evaluated by flow cytometric analysis. Production of cytokines IL-10, transforming growth factor-β, and vascular endothelial growth factor in serum was measured using enzyme-labeled immunosorbent assay kits. The mRNA levels of Foxp3, IL-10, and transforming growth factor-β in the spleen and vascular endothelial growth factor in tumor tissue were detected by real-time PCR. The results showed that triptolide significantly decreased the proportion of regulatory T cells and lowered the Foxp3 level in the spleen and axillary lymph nodes of tumor-bearing mice. Production of IL-10 and transforming growth factor-β in peripheral blood, and the mRNA level of IL-10 and transforming growth factor-β in the spleen were also decreased. Additionally, triptolide could remarkably inhibit production of vascular endothelial growth factor in tumor-bearing mice. In conclusion, our study demonstrated that triptolide might inhibit tumor growth by inhibiting regulatory T cells and some cytokines such as IL-10 and transforming growth factor-β, as well as production of vascular endothelial growth factor. © Georg Thieme Verlag KG Stuttgart.

Keywords

Celastraceae, cytokines, melanoma, regulatory T cells, Tripterygium wilfordii, triptolide

Publication Date

2013

Source Publication Title

Planta Medica

Volume

79

Issue

15

Start Page

1401

End Page

1407

Publisher

Georg Thieme Verlag

ISSN (print)

00320943

ISSN (electronic)

14390221

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