Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

Background: Citrus fruits are widely used as food and or for medicinal purposes, and they contain a host of active substances that contribute to health. The immature fruits of Citrus sinensis Osbeck and its cultivars (CS), C. junos Sieb. ex Tanaka (CJ), C. aurantium L. and its cultivars (CA) and Poncirus trifoliate Raf. (PT) are the most commonly used medicinal herbs in Traditional Chinese Medicine, called Zhishi. And their mature fruits can be used as food.Results: In this study, the essential oils of four different Zhishi species were extracted by steam distillation and detected using gas chromatography- mass spectrometry (GC-MS). A total of 39 volatiles from the four species were tentatively identified. The limonene was the most abundant amongst the four species. Principal component analysis (PCA) of essential oils showed a clear separation of volatiles among CS, CJ and PT. However, CA could not be separated from these three species. Additionally, the volatiles accounting for the variations among the widely separated species were characterized through their corresponding loading weight.Conclusion: Sesquiterpenes were identified as characteristic markers for PT. The content of some monoterpenes could be as taxonomic markers between CS and CJ. This work is of great importance for the evaluation and authentication of Zhishi samples through essential oils.

Keywords

Citrus fruits, Essential oils, GC-MS, PCA, Zhishi

Publication Date

4-2014

Source Publication Title

Chemistry Central Journal

Volume

8

Start Page

22

Publisher

Chemistry Central

Peer Reviewed

1

Copyright

© Liu et al.; licensee Chemistry Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

Funder

This study was financially supported by the National Science Foundation of China (Project No. 30772726, No. 30825047 and No. 81001623).

DOI

10.1186/1752-153X-8-22

Link to Publisher's Edition

http://dx.doi.org/10.1186/1752-153X-8-22

ISSN (print)

1752153X

ISSN (electronic)

1752153X

Additional Files

JA-5213-26093_suppl.doc (666 kB)

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