School of Chinese Medicine
Background: Citrus fruits are widely used as food and or for medicinal purposes, and they contain a host of active substances that contribute to health. The immature fruits of Citrus sinensis Osbeck and its cultivars (CS), C. junos Sieb. ex Tanaka (CJ), C. aurantium L. and its cultivars (CA) and Poncirus trifoliate Raf. (PT) are the most commonly used medicinal herbs in Traditional Chinese Medicine, called Zhishi. And their mature fruits can be used as food.Results: In this study, the essential oils of four different Zhishi species were extracted by steam distillation and detected using gas chromatography- mass spectrometry (GC-MS). A total of 39 volatiles from the four species were tentatively identified. The limonene was the most abundant amongst the four species. Principal component analysis (PCA) of essential oils showed a clear separation of volatiles among CS, CJ and PT. However, CA could not be separated from these three species. Additionally, the volatiles accounting for the variations among the widely separated species were characterized through their corresponding loading weight.Conclusion: Sesquiterpenes were identified as characteristic markers for PT. The content of some monoterpenes could be as taxonomic markers between CS and CJ. This work is of great importance for the evaluation and authentication of Zhishi samples through essential oils.
Citrus fruits, Essential oils, GC-MS, PCA, Zhishi
Source Publication Title
Chemistry Central Journal
© Liu et al.; licensee Chemistry Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
This study was financially supported by the National Science Foundation of China (Project No. 30772726, No. 30825047 and No. 81001623).
Link to Publisher's Edition
Liu, Yuanyan, Zhenli Liu, Chun Wang, Qinglin Zha, Cheng Lu, Zhiqian Song, Zhangchi Ning, Siyu Zhao, Xinmiao Lu, and Aiping Lu. "Study on essential oils from four species of Zhishi with gas chromatography–mass spectrometry." Chemistry Central Journal 8 (2014): 22.