Document Type

Journal Article

Authors

Li-Zhen Zheng, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
Zhong Liu, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
Ming Lei, Department of Orthopaedics and Traumatology, Shenzhen Second People's Hospital
Jiang Peng, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
Yi-Xin He, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
Xin-Hui Xie, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
Chi-Wai Man, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
Le Huang, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
Xin-Luan Wang, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
Daniel Tik-Pui Fong, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong
De-Ming Xiao, Department of Orthopaedics and Traumatology, Shenzhen Second People's Hospital
Da-Ping Wang, Department of Orthopaedics and Traumatology, Shenzhen Second People's Hospital
Yang Chen, Department of Orthopaedics and Traumatology, Shenzhen Second People's Hospital
Jian Q. Feng, Baylor College of Dentistry, University of Missouri-Kansas City
Ying Liu, Baylor College of Dentistry, University of Missouri-Kansas City
Ge Zhang, Department of Orthopaedics and Traumatology, The Chinese University of Hong KongFollow
Ling Qin, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong

Department/Unit

School of Chinese Medicine

Title

Steroid-associated hip joint collapse in bipedal emus

Language

English

Abstract

In this study we established a bipedal animal model of steroid-associated hip joint collapse in emus for testing potential treatment protocols to be developed for prevention of steroid-associated joint collapse in preclinical settings. Five adult male emus were treated with a steroid-associated osteonecrosis (SAON) induction protocol using combination of pulsed lipopolysaccharide (LPS) and methylprednisolone (MPS). Additional three emus were used as normal control. Post-induction, emu gait was observed, magnetic resonance imaging (MRI) was performed, and blood was collected for routine examination, including testing blood coagulation and lipid metabolism. Emus were sacrificed at week 24 post-induction, bilateral femora were collected for micro-computed tomography (micro-CT) and histological analysis. Asymmetric limping gait and abnormal MRI signals were found in steroid-treated emus. SAON was found in all emus with a joint collapse incidence of 70%. The percentage of neutrophils (Neut %) and parameters on lipid metabolism significantly increased after induction. Micro-CT revealed structure deterioration of subchondral trabecular bone. Histomorphometry showed larger fat cell fraction and size, thinning of subchondral plate and cartilage layer, smaller osteoblast perimeter percentage and less blood vessels distributed at collapsed region in SAON group as compared with the normal controls. Scanning electron microscope (SEM) showed poor mineral matrix and more osteo-lacunae outline in the collapsed region in SAON group. The combination of pulsed LPS and MPS developed in the current study was safe and effective to induce SAON and deterioration of subchondral bone in bipedal emus with subsequent femoral head collapse, a typical clinical feature observed in patients under pulsed steroid treatment. In conclusion, bipedal emus could be used as an effective preclinical experimental model to evaluate potential treatment protocols to be developed for prevention of ON-induced hip joint collapse in patients. © 2013 Zheng et al.

Publication Date

2013

Source Publication Title

PLoS ONE

Volume

8

Issue

10

Publisher

Public Library of Science

DOI

10.1371/journal.pone.0076797

ISSN (print)

19326203

ISSN (electronic)

19326203

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