Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Anti-fibrotic and anti-tumorigenic effects of rhein, a natural anthraquinone derivative, in mammalian stellate and carcinoma cells

Language

English

Abstract

© 2014 John Wiley & Sons, Ltd. Anthraquinone compounds have been recognized to possess antiinflammatory, anti-fibrotic and anti-tumour properties and thus applied in human and veterinary therapeutics as active substances of medicinal products. Amongst the anthraquinones isolated from Rheum palmatum, also known as da-huang, rhein was detected as one of the highest metabolite contents in the bloodstream of mammals. The biological activities of rhein therefore deserve detailed investigation. In this study, we aimed to delineate the mechanism of inhibitory actions of rhein on fibrotic and tumorigenic processes by means of various biochemical assays, such as immunofluorescent staining, real-time polymerase chain reaction (PCR) and western blotting analyses in rat pancreatic stellate cells (LTC-14), human pancreatic ductal adenocarcinoma cells (PANC-1) and human colon carcinoma cells (SW480 and SW620). Our results demonstrated that the application of rhein notably suppressed the mRNA and protein levels of various fibrotic and tumorigenic mediators including alpha-smooth muscle actin, type I collagen, fibronectin, N-cadherin and matrix metalloproteinases in the testing mammalian cells. The mechanism of the suppressive actions of rhein was associated with the modulation of the sonic hedgehog and serine-threonine kinase signalling pathways. In conclusion, we suggest that rhein may serve as a therapeutic or an adjuvant agent in anti-fibrotic and anti-tumorigenic approaches

Keywords

anthraquinone, anti-fibrotic agent, anti-tumour agent, carcinoma cells, rhein, stellate cells

Publication Date

2015

Source Publication Title

Phytotherapy Research

Volume

29

Issue

3

Start Page

407

End Page

414

Publisher

Wiley

DOI

10.1002/ptr.5266

Link to Publisher's Edition

http://dx.doi.org/10.1002/ptr.5266

ISSN (print)

0951418X

ISSN (electronic)

10991573

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