Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence

Language

English

Abstract

This study presented a capillary zone electrophoresis (CZE) method for the analysis of trace amount of neurotransmitters glutamate (Glu) and aspartate (Asp) in the microdialysates of rat periaqueductal gray matter (PAG). Glu and Asp were derivatized with the fluorescent agent 5-carboxyfluorescein N-succinimidyl ester (CFSE) for the first time and detected by laser-induced fluorescence (LIF) after separation by CZE. The concentration detection limits (S/N = 2) were 6.9 × 10-10 M and 8.1 × 10-10 M for Glu and Asp, respectively. The repeatability (expressed as RSD) of the migration times of CFSE-amino acid were better than 0.5%, and not higher than 1.5% even over the period of 1 month. This method was applied to quantify Glu and Asp in rat PAG microdialysates with the treatment of formalin injection, and the measured basal concentrations of Glu and Asp were (22.4 ± 1.6) × 10-6 M and (0.9 ± 0.1) × 10-6 M, respectively. © 2009 Elsevier B.V. All rights reserved.

Keywords

5-Carboxyfluorescein N-succinimidyl ester, Aspartate, Capillary electrophoresis, Glutamate, Laser-induced fluorescence, Microdialysis

Publication Date

2009

Source Publication Title

Journal of Chromatography B

Volume

877

Issue

27

Start Page

3248

End Page

3252

Publisher

Elsevier

DOI

10.1016/j.jchromb.2009.08.006

ISSN (print)

15700232

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