http://dx.doi.org/10.1007/s00216-009-2655-6">
 

Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection

Language

English

Abstract

Serotonin (5-hydroxytryptamine, 5-HT) plays vital roles in regulating gastrointestinal functions. Thus, the detection of 5-HT in the gastrointestinal tract is of great importance for biomedical research, medical diagnosis, and pharmaceutical therapy. This paper presents a simple, sensitive, and fast method for the quantification of luminally released serotonin in the feces and tissues of the rat proximal colon by means of capillary electrophoresis with laser-induced fluorescence detection. 5-Carboxyfluorescein N-succinimidyl ester was used for precolumn derivatization of serotonin. The optimal separation and detection conditions were obtained with an electrophoretic buffer containing 60 mM borate (pH 8.90) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). The serotonin concentrations in the feces and tissues of proximal colons were analyzed with this method, and the average values of serotonin in the feces samples were 1.951∈±∈0.446 ng/mg (male) and 2.095∈±∈0.533 ng/mg (female) and 1. 397∈±∈0.267 ng/mg in rat proximal colon tissues. The results demonstrate that this method can accurately determine luminally released 5-HT in rats. © 2009 Springer-Verlag.

Keywords

Capillary electrophoresis, Laser-induced fluorescence, Serotonin

Publication Date

2009

Source Publication Title

Analytical and Bioanalytical Chemistry

Volume

393

Issue

8

Start Page

2059

End Page

2066

Publisher

Springer Verlag

ISSN (print)

16182642

ISSN (electronic)

16182650

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