Document Type

Journal Article

Authors

Ben Chung-Lap Chan, State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong
Hua Yu, State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong
Chun-Wai Wong, State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong
Sau-Lai Lui, State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong
Claude Jolivalt, ChimieParisTech, Laboratoire Charles Friedel (LCF)
Carine Ganem-Elbaz, ChimieParisTech, Laboratoire Charles Friedel (LCF)
Jean-Marc Paris, ChimieParisTech, Laboratoire Charles Friedel (LCF)
Barbara Morleo, Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles
Marc Litaudon, Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles
Clara Bik-San Lau, State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong
Margaret Ip, Department of Microbiology, The Chinese University of Hong Kong
Kwok-Pui Fung, State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong
Ping-Chung Leung, State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong
Quan-Bin Han, State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong KongFollow

Department/Unit

School of Chinese Medicine

Title

Quick identification of kuraridin, a noncytotoxic anti-MRSA (methicillin-resistant Staphylococcus aureus) agent from Sophora flavescens using high-speed counter-current chromatography

Language

English

Abstract

Bacterial resistance to antibiotics has become a serious problem of public health that concerns almost all currently used antibacterial agents and that manifests in all fields of their application. To find more antibacterial agents from natural resources is all the time considered as an important strategy. Sophora flavescens is a popularly used antibacterial herb in Chinese Medicine, from which prenylated flavones were reported as the antibacterial ingredients but with a major concern of toxicity. In our screening on the antibacterial activities of various chemicals of this herb, 18 fractions were obtained from 8. g of 50% ethanol extract on a preparative high-speed counter-current chromatography (HSCCC, 1000. ml). The system of n-hexane/ethyl acetate/methanol/water (1:1:1:1) was used as the two-phase separation solvent. A chalcone named kuraridin was isolated from the best anti-MRSA fraction, together with sophoraflavanone G, a known active ingredient of S. flavescens. Their structures were elucidated by analysis of the NMR spectra. Both compounds exhibited significant anti-MRSA effects, compared to baicalein that is a well known anti-MRSA natural product. More important, kuraridin showed no toxicity on human peripheral blood mononuclear cells (PBMC) at the concentration up to 64. μg/ml while sophoraflavanone G inhibited over 50% of cellular activity at 4. μg/ml or higher concentration. These data suggested that opening of ring A of the prenylated flavones might decrease the toxicity and remain the anti-MRSA effect, from a viewpoint of structure-activity relationship. © 2011 Elsevier B.V.

Keywords

Antibacteria, High-speed counter-current chromatography, Kuraridin, MRSA, Sophora flavescens

Publication Date

2012

Source Publication Title

Journal of Chromatography B

Volume

880

Start Page

157

End Page

162

Publisher

Elsevier

DOI

10.1016/j.jchromb.2011.11.039

ISSN (print)

15700232

This document is currently not available here.

Share

COinS