School of Chinese Medicine
Isolation and structural characterization of an immunostimulating polysaccharide from fuzi, Aconitum carmichaeli
A water-soluble polysaccharide named as FPS-1 was isolated from fuzi, the root of Aconitum carmichaeli Debx. by hot-water extraction, anion-exchange and gel-permeation chromatography and tested for its pharmacological activities. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, methylation analysis and GLC-MS. Based on the data obtained, FPS-1 was determined to be an α-(1→6)-d-glucan, with a weight-average molecular weight of about 14,000 Da. The glucan is highly branched with a single glucose at the C-3 position every four residues, on average, along the main chain. In immunopharmacological studies, FPS-1 showed potent stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide both in vitro and in vivo as well as on splenocyte antibody production. © 2005 Elsevier Ltd. All rights reserved.
α-D-Glucan, Aconitum carmichaeli, Fuzi, Lymphocyte proliferation, Ranunculaceae, Structure
Source Publication Title
Zhao, Chi, Min Li, Yifan Luo, and Weikang Wu. "Isolation and structural characterization of an immunostimulating polysaccharide from fuzi, Aconitum carmichaeli." Carbohydrate Research 341.4 (2006): 485-491.