http://dx.doi.org/10.1016/j.carres.2005.11.032">
 

Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Isolation and structural characterization of an immunostimulating polysaccharide from fuzi, Aconitum carmichaeli

Language

English

Abstract

A water-soluble polysaccharide named as FPS-1 was isolated from fuzi, the root of Aconitum carmichaeli Debx. by hot-water extraction, anion-exchange and gel-permeation chromatography and tested for its pharmacological activities. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, methylation analysis and GLC-MS. Based on the data obtained, FPS-1 was determined to be an α-(1→6)-d-glucan, with a weight-average molecular weight of about 14,000 Da. The glucan is highly branched with a single glucose at the C-3 position every four residues, on average, along the main chain. In immunopharmacological studies, FPS-1 showed potent stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide both in vitro and in vivo as well as on splenocyte antibody production. © 2005 Elsevier Ltd. All rights reserved.

Keywords

α-D-Glucan, Aconitum carmichaeli, Fuzi, Lymphocyte proliferation, Ranunculaceae, Structure

Publication Date

2006

Source Publication Title

Carbohydrate Research

Volume

341

Issue

4

Start Page

485

End Page

491

Publisher

Elsevier

ISSN (print)

00086215

ISSN (electronic)

1873426X

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