School of Chinese Medicine
Saikosaponin-d inhibits T cell activation through the modulation of PKCθ, JNK and NF-κB transcription factor
The effects of saikosaponin-d, a triterpene saponin derived from Bupleurum falcatum L. (Umbelliferae), on the signaling pathways of T cell activation were examined. The results showed that saikosaponin-d potently suppressed both early (CD69) and late (CD71) expressions of mouse T cells stimulated with Con A or PMA. It interfered with PKCθ translocation from cytosol to membrane fraction and inhibited the phosphorylations of IκBα and JNK, but not ERK, in PMA-activated mouse T cells. Additionally, it inhibited PMA and ionomycin-stimulated IL-2 production in mouse T cells. In summary, these results indicate that the mechanism by which saikosaponin-d inhibits T cell activation would involve the suppression of CD69 and CD71 expressions and IL-2 production, and the modulation of PKC pathway through PKCθ, JNK, and NF-κB transcription factor. This may herald a novel approach for further studies of saikosaponin-d as a candidate for use in the treatment of inflammatory and autoimmune diseases. © 2005 Elsevier Inc. All rights reserved.
CD69, CD71, ERK, IκBα, JNK, PKCθ, Saikosaponin-d, Signal transduction, T cell activation
Source Publication Title
Biochemical and Biophysical Research Communications
Leung, Chung Yee, Liang Liu, Ricky N.S. Wong, Yao Ying Zeng, Ming Li, and Hua Zhou. "Saikosaponin-d inhibits T cell activation through the modulation of PKCθ, JNK and NF-κB transcription factor." Biochemical and Biophysical Research Communications 338.4 (2005): 1920-1927.