Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Anti-fibrotic effect of trans-resveratrol on pancreatic stellate cells

Language

English

Abstract

© 2015 Elsevier Masson SAS. Trans-resveratrol, also known as 3,5,4'-trihydroxy-. trans-stilbene, is a natural stilbenoid found at high concentration in skins of red grapes and berries. Over the recent years, it has been reported with a variety of beneficial effects such as antioxidant, anti-aging and anti-inflammatory bioactivities; thus often utilized as an active substance in human and veterinary therapeutics. In the current study, we aimed to delineate the mechanism of its anti-fibrotic action by means of various biochemical assays, such as immunofluorescent staining, real-time polymerase chain reaction and Western blotting analyses in a cellular model, the LTC-14 cells, which retain essential characteristics and morphological features of primary pancreatic stellate cells (PSCs). Our results demonstrated that the application of trans-resveratrol as low as 10. μM notably suppressed the mRNA and protein levels of different fibrotic mediators namely alpha-smooth muscle actin, type I collagen and fibronectin in the LTC-14 cells stimulated with transforming growth factor-beta, a well recognized pro-fibrotic inducer. Importantly, the mechanism of the anti-fibrotic action of trans-resveratrol was associated with a decrease in nuclear factor-kappaB activation and protein kinase B phosphorylation. In conclusion, our finding suggests that trans-resveratrol may serve as a therapeutic or an adjuvant agent in anti-fibrotic approaches and/or PSC-relating pathologies.

Keywords

Anti-fibrotic agent, Pancreatic fibrosis, Pancreatic stellate cells, Resveratrol, Trans-resveratrol

Publication Date

2015

Source Publication Title

Biomedicine & Pharmacotherapy

Volume

71

Start Page

91

End Page

97

Publisher

Elsevier

DOI

10.1016/j.biopha.2015.02.013

ISSN (print)

07533322

ISSN (electronic)

19506007

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